Fig. 5. Vascular function is impaired in endothelial cell-specific HDAC2 knockout mice. (A) Genotyping shows the presence of Cre recombinase under VE-Cadherin (Cdh5) promoter (top) and floxed HDAC2 (HDAC2^fl/fl) (bottom) in endothelial cell-specific HDAC2 knockout mice (Hdac2-ecKO). (B, C) Intact aortas from HDAC2-ecKO mice were immunoblotted for HDAC2 (**p<0.005 vs wild-type [WT], n=3). (D) Pulse wave velocity in 12-14-week-old HDAC2-ecKO mice and littermate controls (**p<0.005 vs WT, n=8). (E, F) Dose response (D/R) curves show myography of isolated aortic rings from HDAC2-ecKO and littermate control mice in response to acetylcholine and sodium nitroprusside (*p<0.05 vs WT, n=6).